Available Services
In this section, you may browse through all services available across all National Facilities. Use the filter function to filter services by National Facility, Infrastructural Unit or category of service. In addition, you may use the text search function to search across services using key words. Please note the system will search your chosen key word either in the service title or description.
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Sequencing only with NovaSeq 6000 (Illumina)
The National Facility for Genomics will provide sequencing of pools of libraries prepared by the users with the NovaSeq6000 sequencing platform (Illumina).
The NovaSeq 6000 is a high-throughput sequencing platform developed by Illumina. It’s designed to handle a wide range of applications and large-scale genomic projects.
NovaSeq 6000 is commonly used for: Whole Genome Sequencing (WGS), Exome Sequencing (WES), Transcriptome Analysis (bulk RNA-Seq or single-cell RNAseq), Metagenomics, Epigenomics studies, Population Genomics.
Sequencing only with NextSeq 2000 (Illumina)
The National Facility for Genomics will provide sequencing of pools of libraries prepared by the users with the NextSeq2000 sequencing platform (Illumina).
The NextSeq 2000 is a next-generation sequencing platform developed by Illumina. It is designed to offer high-throughput sequencing with flexibility for various applications.
The NextSeq 2000 is commonly used for: Whole Genome Sequencing (WGS), RNA Sequencing (RNA-Seq and single-cell RNA-seq), Targeted Sequencing (amplicon sequencing), Exome Sequencing (WES), Metagenomics, ChIP-Seq and Epigenomics.
directCAGE
Direct Cap Analysis of Gene Expression (direct CAGE) is an high-throughput method which enables genome-wide profiling of transcription start sites (TSS) at single-base resolution, allowing precise mapping of promoter and enhancer initiation events, as well as quantitative measurement of gene expression across different cell types and differentiation states (Delobel, D et al.,2025).
This method is based on the selection of 5′ ends of capped RNAs, (‘cap-trap’, Carninci et al.1996). CAGE tags are mapped to the reference genome to identify TSS and their related promoter regions. The number of CAGE tags mapped at specific locations in the genome provides quantification on promoter activities on a genome-wide scale.
This protocol can be used for the detection of differential gene expression between different cell types (tissue specific expression), different stages of cell differentiation (regulation of cell differentiation), determination of promoter and enhancer usage.