Suspension BLISS (sBLISS – in-suspension Breaks Labeling In Situ and Sequencing) is a genome-wide method for identifying and mapping DNA double-strand breaks (DSBs) at nucleotide resolution (Bouwman et al., 2020).
DSBs are among the most critical forms of DNA damage and play key roles in both physiological and pathological processes, including replication stress, transcription associated fragility, and the cellular response to genotoxic agents such as chemotherapeutics, radiomimetic compounds, genome-editing nucleases, or restriction endonucleases.
sBLISS generates quantitative profiles of both endogenous and induced DSBs in any cell type that can be brought into suspension, and has been successfully applied to cultured cells, organoids, and dissociated tissues. By enabling direct detection of DSBs, sBLISS supports research on DNA repair mechanisms, genome stability, and the effects of genotoxic compounds across diverse biological systems.