Total RNA sequencing is a powerful and widely used molecular biology technique that aims to analyse and quantify the entire transcriptome of a biological sample. The transcriptome represents the complete set of RNA molecules, including messenger RNA (mRNA), and non-coding RNAs, present in a cell or tissue. Total RNA sequencing provides a comprehensive view of the transcriptome, allowing researchers to gain insights into gene expression patterns, identify novel transcripts, and understand the regulatory mechanisms underlying various biological processes.