National Facility for Light Imaging

The National Facility for Light Imaging offers services related to imaging, sample preparation, functional imaging and cell sorting. The mission of the facility is to support science: to reach this goal, the facility and its team are available to internal and external users, providing support for designing experiments, acquiring data on the facility’s cutting-edge scientific instruments, offering training and development of new protocols and technologies. Integrated pipelines with other facilities of Human Technopole allow users to access streamlined multi-facility workflows, where data acquired in the National Facility for Light Imaging can be further processed and analyzed by other National Facilities.

Currently, four infrastructural Units (IU) within the National Facility are active and available to external applicants:

  • IU1 – Imaging: The Imaging IU offers access to several high-end microscopy systems. The microscopes can be used to image fixed or living samples across spatial scales, ranging from super-resolution sub-cellular imaging to whole organ imaging. After a mandatory training, users can access the microscopes autonomously. Supervision and assistance from the unit’s staff is available upon request.
  • IU 2 – Tissue Processing: The Tissue Processing IU provides platforms, technical support and training for preparing samples for downstream analysis, such as light imaging or spatial transcriptomics.
  • IU 3 – Flow cytometry applications: The Flow Cytometry IU offers full-service sorting & cloning of rare cellular populations, particle enrichment, and high purity bulk sorts. The unit also offers Analysis Services including combined scalar and image capabilities, and advice in experimental design and training.
  • IU 4 – High-content imaging: Service under construction
  • IU 5 – Ion Imaging: The Ion Imaging IU offers functional cell imaging services using fluorescence-based time-lapse recordings of intracellular ion oscillations or optics-free extracellular voltage recordings through microelectrode arrays (MEA) systems. Optical imaging is primarily based on confocal or epifluorescence microscopy
  • IU 6 – Technology Development – custom microscopy: Service under construction

Open Call

Deadline: 31 December 2024

24-LI-PILOT - National Facility for Light Imaging

The pilot call for Access for the National Facility for Light Imaging (Call ID: 24-LI-PILOT) will be open from the 10th of June 2024 to the 31st of December 2024 and will involve 2 rounds of evaluation (September 2024 and January 2025).

Download the 24-LI-PILOT open call in pdf.


For general enquiries about the call:

For technical enquiries about the services:





  • Leica Thunder Imager Live Cell
  • Zeiss Axioscope5
  • Zeiss Axiozoom V16 with apotome


  • Zeiss LSM980
  • Zeiss LSM980-NLO
  • Nikon Ti2 with CREST v3 spinning disk scan head
  • Leica Stellaris 8 for live-cell imaging


  • Zeiss Elyra7
  • Abberrior FacilityLine
  • Leica Stellaris 8 for super-resolution imaging


  • Zeiss Lattice Lightsheet 7

Slide Scanner

  • Zeiss Axioscan Z.1 automated slide scanner

Flow Cytometry Cell Sorting

  • BD FACSDiscover S8
  • MoFlo Astrios EQ

Flow Cytometry Analysis

  • Cytek Aurora Spectral Analyzer
  • ImageStream Mark II Imaging Flow Cytometer
  • CytoFLEX LX Flow Cytometer

Assisted Flow Cytometry Analysis

Consultation session: Sample Preparation/Panel Design/Data Analysis

Ion imaging assisted experiment

  • MEA assay
  • Ion Imaging assay


IU1 Imaging Arrow right

IU2 Tissue Processing Arrow right

IU3 Flow Cytometry Arrow right

IU5 Ion Imaging Arrow right

Facility Members