National Facility for Genomics

The National Genomics Facility offers cutting-edge and innovative services in the field of genomics. Our mission is to develop robust experimental and analytical workflows to explore all major areas of genomic research. These include, but are not limited to, DNA and RNA analysis, study of chromatin structure, and the epigenetic mechanisms that regulate transcription.

Our goal is to utilize and apply advanced and innovative technologies to provide high-level analyses, supporting large-scale projects, population studies, tissue-level analyses, and single-cell studies. The National Genomics Facility is committed to enhancing genomic research in all its aspects, benefiting the entire Italian scientific community.

The National Genomics Facility is composed of four Infrastructure Units (IU):

  • IU1 – High-throughput Sequencing: The primary goal of this Unit is to provide highly innovative high-throughput sequencing services. Specialized in high-quality genomic, transcriptomic, epigenomic and metagenomic analyses, it utilizes advanced protocols for sample processing with automated systems and next-generation sequencing (NGS) platforms.
  • IU2 – Multi-omics Technologies: This Unit specializes in multi-omics technologies, offering cutting-edge services for single-cell multi-omics analyses and spatial transcriptomics, as well as sequencing based on long-read generation. It closely collaborates with the Tissue Processing Unit and the National Imaging Facility to implement spatial transcriptomics protocols.
  • IU3 – Computational Genomics: Representing the computational core of the Facility, this Unit is dedicated to the development, implementation, and maintenance of automated pipelines for pre-processing and primary analysis of sequencing data. It works in close collaboration with the Data Handling and Data Analysis Facility, ensuring that the computational pipelines are state-of-the-art and tailored to the continuously evolving technological landscape of genomic research.
  • IU4 – Technology Development: The team in this Unit is committed to the development of innovative methods and technologies. With highly qualified personnel, it focuses on evaluating new technologies and tools for optimizing and standardizing customized experimental protocols. The goal is to offer new innovative services to the scientific community and support the realization of proof-of-concept projects to promote technological development and transfer.

Open Call

Deadline: 31 December 2024

24-G-PILOT - National Facility for Genomics

The pilot call for Access for the National Facility for Genomics (Call ID: 24-G-Pilot) will be open from the 10th of June 2024 to the 31st of December 2024 and will involve 2 rounds of evaluation (September 2024 and January 2025).

Download the 24-G-PILOT open call in pdf.


For general enquiries about the call:

For technical enquiries about the services:




  • Whole Genome Sequencing (WGS)
  • Whole Exome Sequencing (WES)
  • Amplicon sequencing for microbiome analysis (16S-ITS)
  • mRNA sequencing from standard and low input
  • totalRNA sequencing from standard input
  • smallRNA sequencing
  • Single-cell 3’RNAsequencing or Single-cell gene Expression Flex (10X Genomics)
  • Single-cell Immune profiling-VDJ (10X Genomics)
  • Single-cell multiome ATAC + Gene expression (10X Genomics)
  • Single-cell ATAC sequencing (10X Genomics)
  • Visium Spatial gene expression from FreshFrozen or FFPE tissues (10X Genomics)
  • Nanopore gDNA sequencing (long reads or ultra long reads)
  • Nanopore small gDNA sequencing
  • Nanopore direct RNA Sequencing
  • Nanopore cDNA sequencing (bulk cDNA or single-cell cDNA from 10x Genomics protocol)
  • Sequencing only with NovaSeq 6000 (Illumina)
  • Sequencing only with NextSeq 2000 (Illumina)
  • Sequencing only with MiSeq (Illumina)


Technologies for Automated Samples/Libraries preparation Arrow right

Technologies for High Throughput Sequencing Arrow right

Technologies for Single-cell RNA Sequencing and Spatial Multiomics Arrow right

Facility Members