RNA sequencing is a powerful molecular biology technique used to analyse the transcriptome of a biological sample. The transcriptome refers to the complete set of RNA molecules, in particular messenger RNA (for mRNA sequencing) and/or non-coding RNAs (for total RNA sequencing), in a cell or tissue.
RNA sequencing is widely used in genomics research, functional genomics, and clinical studies to understand gene expression patterns, identify novel transcripts, and investigate how gene expression varies under different conditions. In addition, total RNA sequencing provides an insight also on the regulatory mechanisms underlying various biological processes.