Available Services
In this section, you may browse through all services available across all National Facilities. Use the filter function to filter services by National Facility, Infrastructural Unit or category of service. In addition, you may use the text search function to search across services using key words. Please note the system will search your chosen key word either in the service title or description.
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Visium Spatial gene expression from Fresh-Frozen or FFPE tissues (10X Genomics)
The Visium Spatial Gene Expression solution by 10X Genomics enables spatial profiling of gene expression in intact tissue sections, preserving cellular spatial context. For fresh-frozen tissues, the protocol involves sectioning, mounting, fixation, permeabilization, reverse transcription, library preparation, sequencing, and spatial mapping. For FFPE tissues, additional steps include deparaffinization, rehydration, antigen retrieval, and tissue mounting with Visium Cytassist. Both protocols end with library preparation, sequencing, data analysis, and spatial mapping to retain spatial information.
