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Nanopore sequencing of small gDNAs will be performed with the native barcoding kit that refers to a set of reagents and protocols designed to enable the simultaneous sequencing of multiple samples by adding unique barcodes to each sample before sequencing. This approach is particularly useful for studying small bacteria genomes (gDNA) as it allows for high-throughput sequencing and analysis of multiple samples using a single flowcell.

This approach provides a cost-effective and efficient way to sequence multiple small bacteria genomes simultaneously, making it a valuable tool in microbiome studies, environmental monitoring, and other applications in microbial genomics.

Nanopore sequencing is a next-generation sequencing technology that uses nanopores to directly sequence DNA molecules and to analyse DNA bases modifications. This method is known for producing long reads, and in some cases, ultra-long reads, making it valuable for various genomic applications.

Direct RNA Sequencing with Nanopore technology is a cutting-edge method for sequencing RNA molecules without the need for conversion to complementary DNA (cDNA) as required in traditional RNA sequencing methods. The protocol involves the direct sequencing of RNA strands through nanopores, allowing for the real-time detection of RNA sequences and RNA bases modifications. Direct RNA Sequencing with Nanopore technology offers the advantage of studying RNA molecules in their native state, providing valuable insights into RNA processing, alternative splicing, and modifications without the need for reverse transcription. It is particularly valuable for capturing the full complexity of the transcriptome.

Cell-free DNA (cfDNA) sequencing with Nanopore technology represents a revolutionary approach to interrogate the genetic information present in circulating DNA, often extracted from blood plasma. Unlike traditional sequencing methods, this protocol allows for the direct sequencing of cfDNA without the need for intermediate steps such as PCR amplification or conversion and allows the analysis in real time of the methylation status of circulating cfDNA. Its applications extend to clinical diagnostics, providing valuable information for personalized medicine and disease monitoring such as liquid biopsy for cancer detection, monitoring treatment response, and identifying minimal residual disease.

Nanopore cDNA sequencing is a powerful technique that combines the benefits of nanopore sequencing technology with the study of complementary DNA (cDNA), which represents the transcribed RNA in a biological sample. This method allows researchers to investigate gene expression, alternative splicing, and other aspects of RNA biology with long-read sequencing capabilities. Nanopore cDNA sequencing offers several advantages, including the ability to generate long reads that span entire transcripts. This makes it particularly valuable for studying complex transcriptomes, characterizing novel isoforms, and exploring the dynamics of gene expression in various biological contexts.