Available Services
In this section, you may browse through all services available across all National Facilities. Use the filter function to filter services by National Facility, Infrastructural Unit or category of service. In addition, you may use the text search function to search across services using key words. Please note the system will search your chosen key word either in the service title or description.
Important: to activate the search remember to click on the magnifying glass icon.
Reprogramming of PBMCs
iPSCs are generated from Peripheral Blood Mononuclear Cells (PBMCs) using footprint-free reprogramming Sendai virus technology.
Every iPSC line undergoes thorough testing to ensure quality and consistency:
- Cell identity confirmation using STR analysis
- Sendai virus clearance
- Expression of undifferentiated stem cell markers
- Assessment of pluripotency markers through 3-germ layer differentiation assay
- Karyotyping (Q-banding) and identification of Copy Number Variations (CNVs) at high resolution
- Master bank post-thaw viability and mycoplasma testing.
Both live and frozen purified PBMCs qualify for the service.
The service typically requires 3 to 4 months for completion and includes the delivery of 1-3 clones, each provided in 10 cryopreserved vials.
Details:
If you’re considering reprogramming other cell types, please contact us for further discussion.
Reprogramming of Fibroblasts
Fibroblasts are reprogrammed using footprint-free, non-modified RNAs (nm-RNA).
Every iPSC line undergoes thorough testing to ensure quality and consistency:
- Cell identity confirmation using STR analysis
- Sendai virus clearance
- Expression of undifferentiated stem cell markers
- Assessment of pluripotency markers upon 3-germ layer differentiation assay
- Karyotyping (Q-banding) and identification of Copy Number Variations (CNVs) at high resolution
- Master bank post-thaw viability and mycoplasma testing.
Both live and frozen fibroblasts at a low passage number (maximum passages 5) qualify for the service.
The service typically requires 3 to 4 months for completion and includes the delivery of 1-3 clones, each provided in 10 cryopreserved vials.
Details:
If you’re considering reprogramming other cell types, please contact us for further discussion.