Available Services

User consultation is an essential first step in assuring high-quality flow cytometric data. Our staff are available to guide new and current Users to meet each projects’ needs, addressing all critical steps between the conception of the flow cytometry experiment, the appropriate steps for sample preparation including the required experimental controls, the acquisition of samples and the analysis of the data generated.

Full-service sorting of rare populations from heterogeneous samples, cell cloning (single cell deposition into multi-well plates), particle enrichment, and high purity bulk sorts.

High-recovery and indexed single-cell sorting for sequencing. Cell sorting of many cell types including:

• immune cell and hematopoietic stem cell subsets
• mesenchymal stem cells
• viable cytokine producing cells
• general cell sorting approaches for cell lines
• transfected cells, including iPSCs Sorting Technical Details

Capable of standard and high-speed sorting of up to 6 populations simultaneously. Sorted cells may be recovered in numerous devices including:

• 6, 12, 24, 28, 96, 384, 1536 well plates & 96 deep well plates
• 2, 0.5, 1.2, 1.5, 2, 5 mL tubes for 6-way sorting
• 15, 50 mL tubes for 2- or 3-way sorting
• Slides or Ibidi vessels
• Custom vessels may also be

The sorter is equipped with 6 lasers having the following emissions: 355 nm 405 nm, 488 nm, 560 nm, 592 nm, 645 nm.

Proper controls for each session must be included with the analysis samples. For example:

• Unstained, unlabeled or other cellular controls
• Spectral overlap compensation controls
• FMO/FMX controls when appropriate

Autonomous Flow Cytometry Analysis (after completion of a training session) or Operator Assisted Flow Cytometry Analysis.

The following are examples of analyses we offer:

• Prepared immune cell and hematopoietic stem cell
• Prepared and labelled samples of mesenchymal stem
• Analysis of cytokine producing
• Multi-color extracellular and intracellular stained
• DNA content analysis (single color or multi-color).
• Cell proliferation
• Apoptosis
• Functional and metabolic assays (mitochondrial function, ROS production, lipid metabolism).
• Analysis of bead assays (e.g., Cytokine/chemokine bead assays). Analyzer Technical Details:
• Integrated absolute count
• Plate loader supporting 96 wells plate and 96 deep wells U, V or Flat bottom
• Different sample injection modes, manual or automatic
• Wide sample flow rate and minimal dead sample volume (about 20 µl)

Analyzers are equipped with 5 or 6 lasers having the following emissions: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm and 808 nm. SSC parameter is available for the 488 and the 405 nm lasers lines.

• Capable of complex polychromatic
• Advice in experimental design and dedicated training sessions are also available. Proper controls for each session must be included with the analysis For example:
• Unstained, unlabeled or other cellular
• Spectral overlap compensation
• FMO/FMX controls when