Available Services

Full-service sorting of rare populations from heterogeneous samples, cell cloning (single cell deposition into multi-well plates), particle enrichment, and high purity bulk sorts.

• High-recovery and indexed single-cell sorting for sequencing.
• Cell sorting of many cell types including immune cell and hematopoietic stem cell subsets, mesenchymal stem cells, viable cytokine producing cells and general cell sorting approaches for cell lines and transfected cells.

Sorter technical features:

• BD FACSDiscover S8
  This instrument is the latest technological advance, representing a breakthrough solution to combine capabilities of cell separation with multiparametric spectral parameter-based analysis and imaging.  Image based cell selection combined with fluorescently labelled tags for determination of tag local. Sorting of up to 6 populations simultaneously with recovery in numerous devices including 384 well microplates. The sorter is equipped with 5 lasers (349 nm, 405 nm, 488 nm, 561 nm, and 637 nm).
• MoFlo Astrios EQ
  The sorter features dual forward scatter for enhanced detection of small particles such as MV’s and capable of very high-speed bulk sorting. Sorting of up to 6 populations simultaneously with recovery in numerous devices including 1536 well microplates. The sorter is equipped with 6 lasers (355 nm, 405 nm, 488 nm, 561 nm, 592 nm, and 640 nm).

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Analysis of cell populations in suspension using conventional, spectral and imaging analyzers.

Analyzer technical features:

• Cytek Aurora Spectral Analyzer
  The analyzer is designed for high-complexity applications, enabling the use of a wide array of new fluorochrome combinations and capable of resolving challenging dye combinations as well as extracting autofluorescence to enhance resolution of dim markers.  The analyzer is equipped with 5 lasers (355 nm, 405 nm, 488 nm, 561 nm, and 640 nm), spectral detection of 64 channels and is equipped with automated sample loader.
• ImageStream Mark II Imaging Flow Cytometer
  This instrument combines the speed, sensitivity, and phenotyping capabilities of flow cytometry with the detailed imagery and functional insights of microscopy. The analyzer is equipped with 6 lasers (375 nm, 405 nm, 488 nm, 561 nm, 592 nm, and 642 nm) and a 785 nm side scatter laser, a 12-channel spectral detector, and multi-channel brightfield.
• CytoFLEX LX Flow Cytometer
  High speed and multi-parametric analysis for immunophenotyping and micro-vesicle analysis with 6 lasers and 21 fluorescence APD detectors. Laser wavelengths include 355, 405, 488, 561, 640, 808 nm. Sample acquisition may be performed in single tubes or in 96-well plates (flat, V or U bottom). Deep-Well plates may also be used for samples requiring lager volumes. In addition to the standard Side Scatter parameter on the 488 nm laser line, an added side-scatter parameter is configured for the 405 nm laser line.

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Autonomous Flow Cytometry Analysis (after completion of a training session) or Operator Assisted Flow Cytometry Analysis.

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