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NATIONAL FACILITY FOR LIGHT IMAGING – IU2 – Tissue Processing

Zeiss Axioscan Z.1 automated slide scanner

The Zeiss Axioscan Z.1 is an automated slide scanner designed to capture whole slide images in both brightfield and epifluorescence modes, accommodating up to 100 slides per session.

Configuration:

  • Cameras:
    • Hamamatsu Orca-Flash 4.0 v3 sCMOS mono camera
    • Hitachi HV-F203SCL 3CCD color camera
  • Light sources:
    • Colibri 7 for epifluorescence (385 nm, 430 nm, 475 nm, 555 nm, 590 nm, 630 nm,

735 nm)

  • White led lamp for transmitted light
  • Filter sets optimised for:
    • DAPI (Zeiss BP 450/40)
    • GFP / Alexa Fluor 488 / FITC (Zeiss BP 525/50)
    • Cy3 / Alexa Fluor 555 / TRITC (Zeiss BP 605/70)
    • Cy5 / Alexa Fluor 647 / APC (Zeiss BP 690/50)
    • DAPI/GFP/Cy3/Cy5/Cy7 (Zeiss PBP 425/30+514/31+592/25+681/45+785/38)
  • 6 objectives:
    • 2,5x / 12 NA Fluar M27
    • 5x / 25 NA Fluar M27
    • 10x / 45 NA Plan-Apochromat M27
    • 20x / 45 NA N-Achroplan Pol M27
    • 20x / 8 NA Plan-Apochromat M27
    • 40x / 95 NA Plan-Apochromat M27
  • Slide racks:
    • Standard microscope slides 75 x 25 mm
    • Macro sections slides 75 x 50 mm
  • File formats:
    • “.czi” native lossless or lossy file format with JpegXR compression
    • “BigTiff” after post processing
    • “OME.TIFF” after post processing

Details:

Image Analysis can be provided as a combined service by the National Facility for Data Handling and Analysis. Please select: SID: NF61.01.01 – Light Microscopy Analysis.

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