Available Services
In this section, you may browse through all services available across all National Facilities. Use the filter function to filter services by National Facility, Infrastructural Unit or category of service. In addition, you may use the text search function to search across services using key words. Please note the system will search your chosen key word either in the service title or description.
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Integrative Modelling with Crosslinking MS and Cryo-EM Data
The Structural Proteomics unit will perform integrative structural modelling combining medium\low resolution (from 7 to 30 Å) cryo-EM densities and crosslinking MS data acquired at our Facility. In case of very large systems, negative stain data may also be used. This does not refer to model building in cryo-EM densities, but to calculations of localization of subunits or of areas not observed in EM maps. This task can be performed on data from services SB-IU4-A or SB-IU4-B only. Example software: DisVis, integrative modelling platform (IMP), AlphaLink\AlphaLink2. This service will be performed by Facility Staff, but training will be available if requested, provided basic bioinformatics skills of the User (bash terminal, python).
Crosslinking MS Acquisition of Purified Protein Complex without Crosslinking Reaction Optimisation
The Structural Proteomics unit will perform crosslinking MS on a purified protein complex to identify protein-protein interactions and map residue distances. This service does not include preliminary crosslinking reaction optimisation, which should be performed by the User at their own institution (in consultation with our Facility Staff). This service will only be performed by Facility Staff.
Crosslinking MS Acquisition of Purified Protein Complex with Crosslinking Reaction Optimisation
The Structural Proteomics unit will perform crosslinking MS on a purified protein complex to identify protein-protein interactions and residue distances. This service includes preliminary crosslinking reaction optimisation and proteomics acquisitions. These can be performed by our Facility Staff provided the protein sample, or by a visiting User with our assistance.
Crosslinking MS Acquisition of Immuno-precipitate From Tagged Overexpressed Bait
The Structural Proteomics unit will perform crosslinking MS to characterize the interactome of a particular target protein in situ. Crosslinking reactions can be performed on cells, in lysate or after competitive or otherwise non-denaturing (native) elution. As this experiment requires a careful and at times tricky design, preliminary results will be shared with the User and guidance provided to set or optimize the experimental conditions. This service will be performed by Facility Staff if the crosslinking reaction occurs at the home institution with guidance from us, or by a visiting User under our direct supervision if the entire workflow is requested.
Crosslinking MS Acquisition of Immuno-precipitate From Endogenous Material Or of Cellular Fraction
The Structural Proteomics unit will perform crosslinking MS to characterize the topology of the protein-protein interactome of a particular target protein or enriched cellular fraction in situ. Examples include: interactome of pulldowns with an endogenously tagged protein of interest; interactome of vesicles\cellular compartments; interactome of bacterial cells or virus\host interactions after pulldown of specific virulence factors. Crosslinking reactions can be performed on cells, on lysates or after competitive or otherwise non-denaturing (native) elution. As these experiments require careful design, the User will be granted access to preliminary acquisitions to characterise the sample and set the correct reaction conditions, or guidance to perform the optimization at their home institution. This service will be performed by our Facility Staff in collaboration with a visiting User.