Available Services

Zeiss LSM980-NLO confocal microscope with multiphoton excitation and Airyscan2 detector.

• Multiphoton excitation: Coherent Discovery NX (dual line, tunable 700-1300 nm and fixed 1040 nm)
• Available laser sources: 405 nm, 488 nm, 561 nm, 594 nm, 639 nm
• Non-descanned detectors
• Airyscan2 detector
• Incubator (CO₂, temperature)
• Epifluorescence (sCMOS camera Zeiss 702)
• Zen Blue version 9
• Available objectives:
• 5x/0.085 EC Plan-Neofluar
• 10x/0.45 Plan-Apochromat
• 20x/0.8 Plan-Apochromat DIC II
• 40x/1.4 Oil Plan-Apochromat
• 40x/1.1 Water C-ApoCHROMAT
• 63x/1.4 Oil PlanApo DIC II

Zeiss LSM980 confocal microscope with Airyscan2 detector.

• Available laser sources: 405 nm, 488 nm, 561 nm, 594 639 nm
• Airyscan2 detector
• Sample finder
• Incubator (CO₂, temperature)
• Epifluorescence (sCMOS camera Zeiss 702)
• Zen Blue version 9
• Available objectives:
  • 5x/0.12 Fluar
  • 10x/0.45 Plan-Apochromat
  • 20x/0.8 Plan-Apochromat
  • 40x/1.4 Oil Plan-Apochromat
  • 63x/1.4 Oil Plan-Apochromat

Zeiss Lattice Lightsheet 7 with dual cameras.

• Available lasers sources: 488 nm, 561 nm and 640 nm
• Dual cameras (Hamamatsu ORCA Fusion)
• Incubator (CO₂, temperature)
• Zeiss Zen Blue software (3.10)

Zeiss Elyra7 lattice structured illumination and localization microscopy system.

• Available laser sources: 405 nm, 488 nm, 561 nm, 638 nm
• Dual camera (PCO.EDGE)
• Incubator (CO₂, temperature)
• Dual computer with Zen black 0 SR for image acquisition and Zen Blue 3.7 for processing
• Available objectives:
• 10x/0.3 EcPlan DIC I
• 20x/0.8 PlanApo DIC II 40x/1.2 W Apo DIC III
• 63x/1.4 Oil PlanApo DIC I
• 63x/1.46 aPlanApo DIC III
• 100x/1.57 aPlanApo DIC III

The Zeiss Axioscan Z.1 is an automated slide scanner designed to capture whole slide images in both brightfield and epifluorescence modes, accommodating up to 100 slides per session.

Configuration:

• Cameras:
  • Hamamatsu Orca-Flash 4.0 v3 sCMOS mono camera
  • Hitachi HV-F203SCL 3CCD color camera
• Light sources:
  • Colibri 7 for epifluorescence (385 nm, 430 nm, 475 nm, 555 nm, 590 nm, 630 nm,

735 nm)

• White led lamp for transmitted light

• Filter sets optimised for:
  • DAPI (Zeiss BP 450/40)
  • GFP / Alexa Fluor 488 / FITC (Zeiss BP 525/50)
  • Cy3 / Alexa Fluor 555 / TRITC (Zeiss BP 605/70)
  • Cy5 / Alexa Fluor 647 / APC (Zeiss BP 690/50)
  • DAPI/GFP/Cy3/Cy5/Cy7 (Zeiss PBP 425/30+514/31+592/25+681/45+785/38)
• 6 objectives:
  • 2,5x / 12 NA Fluar M27
  • 5x / 25 NA Fluar M27
  • 10x / 45 NA Plan-Apochromat M27
  • 20x / 45 NA N-Achroplan Pol M27
  • 20x / 8 NA Plan-Apochromat M27
  • 40x / 95 NA Plan-Apochromat M27
• Slide racks:
  • Standard microscope slides 75 x 25 mm
  • Macro sections slides 75 x 50 mm
• File formats:
  • “.czi” native lossless or lossy file format with JpegXR compression
  • “BigTiff” after post processing
  • “OME.TIFF” after post processing

Nikon Ti2 inverted microscope with CrestOptics X-light V3 spinning disk scan head.

• Available lasers sources: 405 nm, 446 nm, 477 nm, 520 nm, 547 nm, 638 nm, 749 nm
• Available laser sources (TIRF and FRAP): 405 nm, 488 nm, 561 nm, 640 nm
• Four high-speed, back sided illuminated sCMOS cameras (Prime 95B, 25 mm FOV)
• X-light V3 spinning disk scan head (50 µm pinhole disk)
• Incubator (CO₂, temperature)
• TIRF condenser
• Photomanipulation module
• NIS elements software
• Available objectives:
• 4x/0.20 Plan Apo
• 10x/0.45 lD CFI Plan Apochromat
• 20x/0.8 lD CFI Plan Apochromat
• 20x/0.45 S Plan Fluor ELWD
• 40x/1.25 Sil lD CFI Plan Apochromat
• 100x/1.35 Sil lS CFI SR HP Plan Apochromat
• 100x/1.49 Oil CFI Apo TIRF

High resolution, functional imaging based on high density MEA electronic interfaces measuring extracellular voltage potentials. The system available is the multichannel amplifier BioCAM DupleX and planar MEAs electronic chips equipped with 4,096 electrodes (3BRAIN AG, Pfäffikon SZ, Switzerland). The MEAs chip is the CorePlate^TM 1W38/60 (NF55.004_MEA chip testing and provision) with a recording area of 3.8 mm x 3.8 mm, each electrode is 21 mm x 21 mm with a 60 mm pitch. The assay is suitable for the characterization of functional phenotypes in cell networks, and for compound screening. The high density of electrodes allows to collect a large amount of data within a single recording, and it is particularly suitable for accessing the electrical phenotype of brain and cardiac organoids.

Leica Thunder motorized epifluorescence microscope with integrated digital clearing.

• Available LEDs: 395 nm, 438 nm, 475 nm, 551 nm, 555 nm, 575 nm, 635 nm and 730 nm
• Incubator (CO₂, temperature)
• Leica K8 sCMOS camera
• Leica LAX software
• Leica Navigator
• Computational Clearing module
• Available objectives
• 4x/0.10 HI PLAN
• 10x/0.32 HC PL FLUOTAR PH1
• 20x/0.40 Korr HC PL FLUOTAR L PH1
• 40x/0.60 Korr HC PL FLUOTAR PH2
• 63x/1.40-0.60 HC PL APO Oil

Leica Stellaris 8 confocal microscope with tandem scanners (resonant and conventional galvanometer scanner), STED and FALCON modules.

• Available lasers sources: 405 nm laser and tunable white light laser with up to 8 simultaneous lines between 440 nm and 790 nm
• 4 hybrid detectors
• STED module with 775 nm depletion laser
• FALCON module for fluorescence lifetime imaging
• Incubator (CO₂, temperature)
• Leica LAX software
• Leica Navigator
• Available objectives:
• 10x/0.40 HC PL APO CS2
• 20x/0.75 HC PL APO CS2
• 40x/1.30 HC PL APO Oil CS2
• 63x/1.40 HC PL APO Oil CS2
• 100x/1.40 HC PL APO Oil STED

Leica Stellaris 8 confocal microscope with tandem scanners (resonant and conventional galvanometer scanner), incubator and FALCON module.

• Available lasers sources: 405 nm laser and tunable white laser with up to 8 simultaneous lines between 440 nm and 790 nm
• 4 hybrid detectors
• Incubator (CO₂, temperature)
• FALCON module for fluorescence lifetime imaging
• Leica LAX software
• Leica Navigator
• Available objectives:
• 10x/0.40 HC PL APO CS2
• 20x/0.75 HC PL APO CS2
• 40x/1.30 HC PL APO Oil CS2
• 63x/1.40 HC PL APO Oil CS2

Fluorescence-based time-lapse recordings of intracellular ion oscillations, based on confocal or epifluorescence microscopy.

• Imaging on Nikon Ti2 spinning disk with four cameras, TIRF and FRAP module (SID: 007 or equivalent system suggested by the NF staff).

User consultation is an essential first step in assuring high-quality flow cytometric data. Our staff are available to guide new and current Users to meet each projects’ needs, addressing all critical steps between the conception of the flow cytometry experiment, the appropriate steps for sample preparation including the required experimental controls, the acquisition of samples and the analysis of the data generated.

Full-service sorting of rare populations from heterogeneous samples, cell cloning (single cell deposition into multi-well plates), particle enrichment, and high purity bulk sorts.

High-recovery and indexed single-cell sorting for sequencing. Cell sorting of many cell types including:

• immune cell and hematopoietic stem cell subsets
• mesenchymal stem cells
• viable cytokine producing cells
• general cell sorting approaches for cell lines
• transfected cells, including iPSCs Sorting Technical Details

Capable of standard and high-speed sorting of up to 6 populations simultaneously. Sorted cells may be recovered in numerous devices including:

• 6, 12, 24, 28, 96, 384, 1536 well plates & 96 deep well plates
• 2, 0.5, 1.2, 1.5, 2, 5 mL tubes for 6-way sorting
• 15, 50 mL tubes for 2- or 3-way sorting
• Slides or Ibidi vessels
• Custom vessels may also be

The sorter is equipped with 6 lasers having the following emissions: 355 nm 405 nm, 488 nm, 560 nm, 592 nm, 645 nm.

Proper controls for each session must be included with the analysis samples. For example:

• Unstained, unlabeled or other cellular controls
• Spectral overlap compensation controls
• FMO/FMX controls when appropriate

Autonomous Flow Cytometry Analysis (after completion of a training session) or Operator Assisted Flow Cytometry Analysis.

The following are examples of analyses we offer:

• Prepared immune cell and hematopoietic stem cell
• Prepared and labelled samples of mesenchymal stem
• Analysis of cytokine producing
• Multi-color extracellular and intracellular stained
• DNA content analysis (single color or multi-color).
• Cell proliferation
• Apoptosis
• Functional and metabolic assays (mitochondrial function, ROS production, lipid metabolism).
• Analysis of bead assays (e.g., Cytokine/chemokine bead assays). Analyzer Technical Details:
• Integrated absolute count
• Plate loader supporting 96 wells plate and 96 deep wells U, V or Flat bottom
• Different sample injection modes, manual or automatic
• Wide sample flow rate and minimal dead sample volume (about 20 µl)

Analyzers are equipped with 5 or 6 lasers having the following emissions: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm and 808 nm. SSC parameter is available for the 488 and the 405 nm lasers lines.

• Capable of complex polychromatic
• Advice in experimental design and dedicated training sessions are also available. Proper controls for each session must be included with the analysis For example:
• Unstained, unlabeled or other cellular
• Spectral overlap compensation
• FMO/FMX controls when

Abberior Facility Line STED with adaptive optics for deep-tissue super-resolution imaging.

• Available laser sources: 405 nm, 488nm, 561 nm and 640 nm
• Depletion laser: 775 nm
• Adaptive optics system
• Incubator (CO₂, temperature)
• MATRIX detector
• Available objectives:
• UPlanXApo 60x/1.42 Oil
• UPlanSApo 60x/1.20 Water