Fibroblasts are reprogrammed using footprint-free, non-modified RNAs (nm-RNA).
Every iPSC line undergoes thorough testing to ensure quality and consistency:
- Cell identity confirmation using STR analysis
- Sendai virus clearance
- Expression of undifferentiated stem cell markers
- Assessment of pluripotency markers upon 3-germ layer differentiation assay
- Karyotyping (Q-banding) and identification of Copy Number Variations (CNVs) at high resolution
- Master bank post-thaw viability and mycoplasma testing.
Both live and frozen fibroblasts at a low passage number (maximum passages 5) qualify for the service.
The service typically requires 3 to 4 months for completion and includes the delivery of 1-3 clones, each provided in 10 cryopreserved vials.