Reprogramming of PBMCs

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iPSCs are generated from Peripheral Blood Mononuclear Cells (PBMCs) using footprint-free reprogramming Sendai virus technology.

Every iPSC line undergoes thorough testing to ensure quality and consistency:

  • Cell identity confirmation using STR analysis
  • Sendai virus clearance
  • Expression of undifferentiated stem cell markers
  • Assessment of pluripotency markers through 3-germ layer differentiation assay
  • Karyotyping (Q-banding) and identification of Copy Number Variations (CNVs) at high resolution
  • Master bank post-thaw viability and mycoplasma testing.

Both live and frozen purified PBMCs qualify for the service.

The service typically requires 3 to 4 months for completion and includes the delivery of 1-3 clones, each provided in 10 cryopreserved vials.