Nanopore sequencing directly sequences DNA using nanopores, known for producing long or ultra-long reads. Protocol includes DNA extraction, minimal fragmentation for long reads or no fragmentation for ultra-long reads generation, DNA end repair, adapter ligation, device setup, sequencing, and data collection through electrical signals. Base-calling converts signals into DNA sequence. Data analysis involves error correction, read filtering, and read assembly.
The library prep method for long-read sequencing involves repairing DNA ends, dA-tailing, and ligating sequencing adapters. It achieves >99% sequencing accuracy (Q20+) on R10.4.1 flow cells. It is compatible with target enrichment, whole genome amplification, and size selection.
The Ultra-Long DNA Sequencing library prep method facilitates preparation of ultra-high molecular weight DNA, yielding N50s >50 kb and reads up to 4+ Mb. Utilizing transposase chemistry, it cleaves template molecules and attaches tags, followed by rapid adapter addition.