Nanopore cDNA sequencing is a powerful technique that combines the benefits of nanopore sequencing technology with the study of complementary DNA (cDNA), which represents the transcribed RNA in a biological sample. This method allows researchers to investigate gene expression, alternative splicing, and other aspects of RNA biology with long-read sequencing capabilities. Nanopore cDNA sequencing offers several advantages, including the ability to generate long reads that span entire transcripts. This makes it particularly valuable for studying complex transcriptomes, characterizing novel isoforms, and exploring the dynamics of gene expression in various biological contexts.