Integrative Modelling with Crosslinking MS and Cryo-EM Data

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  • Integrative Modelling with Crosslinking MS and Cryo-EM Data

The Structural Proteomics unit will perform integrative structural modelling combining medium\low resolution (from 7 to 30 Å) cryo-EM densities and crosslinking MS data acquired at our Facility. In case of very large systems, negative stain data may also be used. This does not refer to model building in cryo-EM densities, but to calculations of localization of subunits or of areas not observed in EM maps. This task can be performed on data from services SB-IU4-A or SB-IU4-B only. Example software: DisVis, integrative modelling platform (IMP), AlphaLink\AlphaLink2. This service will be performed by Facility Staff, but training will be available if requested, provided basic bioinformatics skills of the User (bash terminal, python).