Negative Stain EM Screening

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  • Negative Stain EM Screening

We offer sample preparation by negative stain EM followed by TEM imaging at 120 kV. A maximum of 1 specimen and 8 grids can be prepared and processed per unit of service. Imaging will be provided for a maximum of 8 continuous hours per unit of service. An optional “polishing” size-exclusion chromatography (SEC) step can be performed on thawed material by the Biophysics Unit, as and if specified in the request for this service. 400 mesh copper grids with amorphous carbon film layer will be used as support. Glow discharging will be performed by a Pelco EasyGlow device. Staining will be performed with a 2% (w\v) uranyl acetate aqueous solution. Imaging in TEM mode at 120 kV will be performed on a Thermo Scientific Talos L120C equipped with CETA 16M camera. Imaging conditions requested by the User, if provided, must be compatible with Facility practices. This service will only be performed by Facility Staff.