Proteomics acquisition on Orbitrap Astral – high load

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  • Proteomics acquisition on Orbitrap Astral – high load

This service comprises 8 hours of equipment time on the Orbitrap Astral LC-MS. The configuration of the system is: Vanquish NEO HPLC in trap and elute configuration. Mobile phase A is 0.1% formic acid in water, mobile phase B is 80% acetonitrile in water with 0.1% formic acid. The MS is set up with an EasySpray source. The MS is operated for high- load, using EasySpray pepMap NEO columns (50cm, 75µm diameter, 10nm pore size). It is possible to use the FAIMS filter to improve sensitivity or fractionate samples online. Overhead on measurements due to sample loading and column washing is about 10 minutes per injection. Typical gradient length 30 minutes (no gains are typically observed beyond this gradient length due to the speed of the Astral analyser). Both DIA and DDA proteomics workflows are available. Each proposal can request up to 9 sessions for a total of 3 days of MS time. The service does not comprise sample preparation from proteins to peptides.