Single-cell RNA sequencing (scRNA-seq) is a technique used to analyse gene expression at the individual cell level, making it possible to resolve cellular heterogeneity within a biological sample. Unlike bulk RNA sequencing, which averages gene expression across many cells, scRNA-seq enables the identification of distinct cell types, states, and subpopulations.
This approach is crucial for understanding complex tissues, developmental processes, and disease progression, as it reveals how gene expression varies from cell to cell, and it is widely applied in biomedical research to advance personalized medicine, immunology, cancer research, and tissue regeneration studies.