Single-cell immune profiling-V(D)J is a powerful molecular biology technique used to profile both 5’ gene expression and T-cell and/or B-cell receptors of individual cells at a high resolution allowing the characterization of cellular heterogeneity and clonal expansion within a biological sample.
Unlike bulk RNA and T/B-cell receptor (TCR/BCR) sequencing, which allow to study gene expression and TCR/BCR repertoires across many cells, single-cell immune profiling-V(D)J enables the identification of distinct cell types, states, and subpopulations both in terms of transcriptional profile (GEX data) and TCR/BCR repertoires (V(D)J data). This approach is crucial for understanding complex tissues, developmental progression, tumorigenesis, and tracking clonal expansion and immune responses. It is widely applied in biomedical research to advance personalized medicine, immunology, cancer immunotherapy, autoimmune disease and infection disease.
The Single-cell Immune profiling-V(D)J datasets include two modalities: gene expression (GEX) and TCR/BCR (V(D)J).