Zeiss Axioscan Z.1 automated slide scanner

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The Zeiss Axioscan Z.1 is an automated slide scanner designed to capture whole slide images in both brightfield and epifluorescence modes, accommodating up to 100 slides per session.

Configuration:

  • Cameras:
    • Hamamatsu Orca-Flash 4.0 v3 sCMOS mono camera
    • Hitachi HV-F203SCL 3CCD color camera
  • Light sources:
    • Colibri 7 for epifluorescence (385 nm, 430 nm, 475 nm, 555 nm, 590 nm, 630 nm,

735 nm)

  • White led lamp for transmitted light
  • Filter sets optimised for:
    • DAPI (Zeiss BP 450/40)
    • GFP / Alexa Fluor 488 / FITC (Zeiss BP 525/50)
    • Cy3 / Alexa Fluor 555 / TRITC (Zeiss BP 605/70)
    • Cy5 / Alexa Fluor 647 / APC (Zeiss BP 690/50)
    • DAPI/GFP/Cy3/Cy5/Cy7 (Zeiss PBP 425/30+514/31+592/25+681/45+785/38)
  • 6 objectives:
    • 2,5x / 12 NA Fluar M27
    • 5x / 25 NA Fluar M27
    • 10x / 45 NA Plan-Apochromat M27
    • 20x / 45 NA N-Achroplan Pol M27
    • 20x / 8 NA Plan-Apochromat M27
    • 40x / 95 NA Plan-Apochromat M27
  • Slide racks:
    • Standard microscope slides 75 x 25 mm
    • Macro sections slides 75 x 50 mm
  • File formats:
    • “.czi” native lossless or lossy file format with JpegXR compression
    • “BigTiff” after post processing
    • “OME.TIFF” after post processing