Gonzalo Alvarez Viar


  • 11/2021 - BioRxiv

    In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

    he transition zone, then as it extends into the cytosol, first the dynein-1b and then the IFTA densities are missing. This suggests the IFTB backbone is built first, followed by IFTA and then dynein-2 recruitment. Kinesin-2 is relatively small and flexible and so could not be identified by cryo-ET. Instead, the authors use expansion microscopy […]

  • 07/2019 - Current Opinion in Structural Biology

    Towards a mechanistic understanding of cellular processes by cryoEM

    A series of recent hardware and software developments have transformed cryo-electron microscopy (cryoEM) from a niche tool into a method that has become indispensable in structural and functional biology. Samples that are rapidly frozen are encased in a near-native state inside a layer of amorphous ice, and then imaged in an electron microscope cooled to […]