05/2024 - Tubulin posttranslational modifications through the lens of new technologies

The Tubulin Code revolutionizes our understanding of microtubule dynamics and functions, proposing a nuanced system governed by tubulin isotypes, posttranslational modifications (PTMs) and microtubule-associated proteins (MAPs). Tubulin isotypes, diverse across species, contribute structural complexity, and are thought to influence microtubule functions. PTMs encode dynamic information on microtubules, which are read by several microtubule interacting proteins and […]

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04/2024 - Predicting bovine daily milk yield by leveraging genomic breeding values

The main goal of this work, conducted on a herd of 502 Holstein cows situated in Italy, is to propose a machine learning-based approach to forecast the individual bovine daily milk production by explicitly leveraging genotypic information. As part of our study, we also evaluated the importance in the prediction of genotypic and phenotypic variables usually available […]

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03/2024 - Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until recently suffered from low integration efficiencies despite traditional genetics being well established. Here, we present a protocol for efficient homology-directed knockin mutagenesis in all commonly used strains of Chlamydomonas. We describe steps for scarless integration of fusion tags and sequence modifications of almost all proteins […]

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02/2024 - Decryption of sequence, structure, and functional features of SINE repeat elements in SINEUP non-coding RNA-mediated post-transcriptional gene regulation

RNA structure folding largely influences RNA regulation by providing flexibility and functional diversity. In silico and in vitro analyses are limited in their ability to capture the intricate relationships between dynamic RNA structure and RNA functional diversity present in the cell. Here, we investigate sequence, structure and functional features of mouse and human SINE-transcribed retrotransposons […]

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