Publications

The main goal of this work, conducted on a herd of 502 Holstein cows situated in Italy, is to propose a machine learning-based approach to forecast the individual bovine daily milk production by explicitly leveraging genotypic information. As part of our study, we also evaluated the importance in the prediction of genotypic and phenotypic variables usually available […]

CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until recently suffered from low integration efficiencies despite traditional genetics being well established. Here, we present a protocol for efficient homology-directed knockin mutagenesis in all commonly used strains of Chlamydomonas. We describe steps for scarless integration of fusion tags and sequence modifications of almost all proteins […]

RNA structure folding largely influences RNA regulation by providing flexibility and functional diversity. In silico and in vitro analyses are limited in their ability to capture the intricate relationships between dynamic RNA structure and RNA functional diversity present in the cell. Here, we investigate sequence, structure and functional features of mouse and human SINE-transcribed retrotransposons […]

In this talk I will show how we combine EM and molecular cell biology methods to obtain a mechanistic understanding of the molecular machineries required for the assembly and the function of cilia/flagella: evolutionary conserved organelles with fundamental roles in human health and disease. Assembly of the cilium requires the rapid bidirectional transport (intraflagellar transport […]