Alessandro Vannini is a biochemist. He heads the Centre for Structural Biology after almost 8 years as a Principal Investigator and Deputy Head of Division at the Institute of Cancer Research in London.
His laboratory focuses on structural and functional analisys of large macromolecular complexes assembling around RNA Pol III loci and that play a role in gene expression and structural organization of the eukaryotic genome. These mechanisms are often deregulated in human diseases, such as cancer and congenital neurodegenerative diseases.
06/2020 - Nature Communications
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability
The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional initiation factor Bdp1 in the RNA polymerase (RNAP) III system, however, remained elusive. A poorly studied aspect in this context is the effect of DNA […]
11/2019 - Molecular Cell
TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation
How repetitive elements, epigenetic modifications, and architectural proteins interact ensuring proper genome expression remains poorly understood. Here, we report regulatory mechanisms unveiling a central role of Alu elements (AEs) and RNA polymerase III transcription factor C (TFIIIC) in structurally and functionally modulating the genome via chromatin looping and histone acetylation. Upon serum deprivation, a subset […]
01/2018 - Nature
RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene transcription by Pol III requires the activity of the transcription factor TFIIIB to form a transcriptionally active Pol III preinitiation […]