Giuseppe Testa

Giuseppe Testa

Giuseppe Testa, MD, PHD, MA, is a professor of Molecular Biology at Milan’s Università Statale and Director of the High Definition Disease Modelling Lab: Stem Cell and Organoid Epigenetics at the European Institute of Oncology. At Human Technopole he heads the Centre for Neurogenomics, within which his lab studies the mechanisms of intellectual disability and autism.

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Publications

  • 10/2022 - BioRxiv

    GTF2I dosage regulates neuronal differentiation and social behavior in 7q11.23 neurodevelopmental disorders

    Copy number variations at 7q11.23 cause neurodevelopmental disorders with shared and opposite manifestations. Deletion causes Williams-Beuren syndrome (WBS), while duplication causes 7q11.23 microduplication syndrome (7Dup). Converging evidence indicates GTF2I, from the 7q11.23 locus, is a key mediator of the cognitive-behavioral phenotypes associated with WBS and 7Dup. Here we integrate molecular profiling of patient-derived cortical organoids (COs) […]

  • 10/2022 - BioRxiv

    7q11.23 CNV alters protein synthesis and REST-mediated neuronal intrinsic excitability

    Copy number variations (CNVs) at 7q11.23 cause Williams-Beuren (WBS) and 7q microduplication syndromes (7Dup), two neurodevelopmental disorders with shared and opposite cognitive-behavioral phenotypes. Using patient-derived and isogenic neurons, we integrated transcriptomics, translatomics and proteomics to elucidate the molecular underpinnings of this dosage effect. We found that 7q11.23 CNVs cause opposite alterations in neuronal differentiation and […]

  • 09/2022 - Nature

    A nomenclature consensus for nervous system organoids and assembloids

    Self-organizing three-dimensional cellular models derived from human pluripotent stem cells or primary tissue have great potential to provide insights into how the human nervous system develops, what makes it unique and how disorders of the nervous system arise, progress and could be treated. Here, to facilitate progress and improve communication with the scientific community and […]

  • 05/2022 - Frontiers in Neuroscience

    EZH2-Mediated H3K27me3 Targets Transcriptional Circuits of Neuronal Differentiation

    The Polycomb Repressive Complex 2 (PRC2) plays important roles in the epigenetic regulation of cellular development and differentiation through H3K27me3-dependent transcriptional repression. Aberrant PRC2 activity has been associated with cancer and neurodevelopmental disorders, particularly with respect to the malfunction of sits catalytic subunit EZH2. Here, we investigated the role of the EZH2-mediated H3K27me3 apposition in […]