Adrian Pascal Nievergelt


Post-doctoral EMBO and HFSP fellowships


  • 09/2022 - Current Biology

    Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport

    Cilia or eukaryotic flagella are microtubule-based organelles found across the eukaryotic tree of life. Their very high aspect ratio and crowded interior are unfavorable to diffusive transport of most components required for their assembly and maintenance. Instead, a system of intraflagellar transport (IFT) trains moves cargo rapidly up and down the cilium (Figure 1A).1, 2, 3 Anterograde IFT, from the cell body to the […]

  • 07/2022 - BioRxiv

    Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas

    CRISPR/Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until now only been applied to targeted gene disruption, whereas scar-less knock-in transgenesis has generally been considered infeasible. We have developed highly efficient homology-directed knock-in mutagenesis in cell-walled strains of Chlamydomonas. Our method allows scarless integration of fusion tags and sequence modifications of near arbitrary proteins […]

  • 07/2019 - Current Opinion in Structural Biology

    Towards a mechanistic understanding of cellular processes by cryoEM

    A series of recent hardware and software developments have transformed cryo-electron microscopy (cryoEM) from a niche tool into a method that has become indispensable in structural and functional biology. Samples that are rapidly frozen are encased in a near-native state inside a layer of amorphous ice, and then imaged in an electron microscope cooled to […]