Alice Giustacchini
- Research Group Leader, Giustacchini Group
Alice ha conseguito il dottorato di ricerca presso l’Università San Raffaele sotto la supervisione del professor Luigi Naldini, con un progetto incentrato sul ruolo dei microRNA nella regolazione delle funzioni delle cellule staminali ematopoietiche. Successivamente si è trasferita nel Regno Unito per intraprendere un Postdoc nei laboratori dei professori Sten Eirik Jacobsen e Adam Mead presso l’Università di Oxford. Durante il suo Postdoc si è concentrata sullo sviluppo di nuovi approcci a singola cellula per risolvere l’eterogeneità cellulare nelle cellule staminali leucemiche durante la risposta alla terapia. Dal 2019, Alice dirige un gruppo di ricerca presso l’University College London (UCL) Great Ormond Street Institute of Child Health e ora è entrata a far parte di Human Technopole per creare un gruppo di ricerca. La ricerca del gruppo di Alice ruota attorno alla comprensione dei meccanismi biologici che rendono la leucemia non responsiva alle terapie convenzionali e all’identificazione di nuovi bersagli terapeutici che consentano di colpirli selettivamente. Combinando la genomica e la proteomica di una singola cellula con saggi funzionali di cellule staminali, il suo gruppo mira a sviluppare nuove strategie per prevenire e trattare la progressione della leucemia.
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Pubblicazioni
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09/2023 - Nature Communications
Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner
Hematopoietic stem cells (HSCs) residing in specialized niches in the bone marrow are responsible for the balanced output of multiple short-lived blood cell lineages in steady-state and in response to different challenges. However, feedback mechanisms by which HSCs, through their niches, sense acute losses of specific blood cell lineages remain to be established. While all […]
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04/2023 - blooad advances
Activation priming and cytokine polyfunctionality modulate the enhanced functionality of low-affinity CD19 CAR T cells
We recently described a low-affinity second-generation CD19 chimeric antigen receptor (CAR) CAT that showed enhanced expansion, cytotoxicity, and antitumor efficacy compared with the high-affinity (FMC63-based) CAR used in tisagenlecleucel, in preclinical models. Furthermore, CAT demonstrated an excellent toxicity profile, enhanced in vivo expansion, and long-term persistence in a phase 1 clinical study. To understand the […]
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03/2022 - STAR Protocols
High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry
Here, we present a comprehensive protocol for the generation and functional characterization of chimeric antigen receptor (CAR) T cells and their products by mass cytometry in a reproducible and scalable manner. We describe the production of CAR T cells from human peripheral blood mononuclear cells. We then detail a three-step staining protocol with metal-labeled antibodies and the […]
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03/2019 - Molecular Cell
Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for resolving transcriptional heterogeneity. However, its application to studying cancerous tissues is currently hampered by the lack of coverage across key mutation hotspots in the vast majority of cells; this lack of coverage prevents the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, […]
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05/2017 - Nature Medicine
Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia
Recent advances in single-cell transcriptomics are ideally placed to unravel intratumoral heterogeneity and selective resistance of cancer stem cell (SC) subpopulations to molecularly targeted cancer therapies. However, current single-cell RNA-sequencing approaches lack the sensitivity required to reliably detect somatic mutations. We developed a method that combines high-sensitivity mutation detection with whole-transcriptome analysis of the same […]
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