Three-dimensional (3D) organisation of the eukaryotic nuclear genome has a crucial role in gene regulation. Magda Bienko and Nicola Crosetto overview emerging and established techniques used to investigate the spatial arrangement of the genome in the nucleus and how it affects gene function in single cells and tissues.
Tight packing into chromatin fibers and their folding into 3D superstructures are necessary to encase meters of DNA in a tiny space such as the nucleus of eukaryotic cells. The 3D arrangement of nuclear DNA fulfils functions far beyond the mere packing of the genome into the nucleus and extends to the regulation of gene replication, expression, and integrity. Therefore, investigating how the genetic material is arranged within the nucleus of a single isolated cell (3D genomics) or in its tissue context (spatial genomics) is instrumental to better understand the mechanism(s) of gene regulation.
Madga Bienko and Nicola Crosetto at the HT Genomics Research Centre and Britta Bouwman (Karolinska Institutet and Science for Life Laboratory, Sweden) review the different methods, for example massively parallel sequencing and high-resolution microscopy among them, which have been used over the past decade to study the 3D organization of the eukaryotic genome. The researchers discuss how these tools may help to address outstanding open questions in the 3D genomics and spatial genomics fields as well as in developmental biology and cancer medicine. Finally, Bienko and Crosetto foresee that the rapid progress in sample preparation protocols, imaging, sequencing, and high-performance computing will allow spatial resolution of both whole-exome and whole-genome sequencing.
The review is now published in Trends in Genetics.
Which brain connections coordinate the sense of sight with movement, for example maintaining stable vision while walking or moving our eyes? How is cholesterol formed and what functions does it have in its intermediate steps? These are the topics of the research projects awarded the Early Career Fellowship Programme, a call for proposals launched by […]
The first symposium on “Gender Equality and Diversity at HT” took place today at the Human Technopole Auditorium. The aim of the event was to discuss why there are differences in the career outcomes of men and women and how we can act to promote equality and inclusion at HT. During the event we heard from Gerlind […]
The Supervisory Board of the Human Technopole Foundation has appointed Marino Zerial as the institute’s new Director. Currently Director of the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany, a research institution he helped found over 25 years ago, Marino Zerial is also an honorary professor at the Faculty of Medicine […]
Magda Bienko, Group Leader at the Functional Genomics Centre at Human Technopole has been awarded an ERC Consolidator Grant for her research project “Radialis”. The project, which has earned a funding of 2 million euros, aims to understand the design principles shaping genome architecture. Within our cells, DNA is folded in a three-dimensional structure. The […]
A web-based application developed by the Iorio Group and the IT & digitalisation team at Human Technopole helps non-computational scientists process and analyse CRISPR-Cas9 screens by reducing false-positive hits. CRISPR/Cas9 technology has become the gold standard for genome and epigenome editing, for studying the regulation of gene expression, and for high-throughput screenings aiming at identifying […]
Manage Cookie Consent
Technical cookies (required)
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.