Ivano Legnini
- Research Group Leader, Legnini Group
Ivano Legnini è un biologo molecolare e dei sistemi interessato alla regolazione genica.
Si è laureato in Genetica e Biologia Molecolare all’Università La Sapienza di Roma nel 2012. Ha lavorato sull’RNA non codificante e la regolazione post-trascrizionale dell’espressione genica nel laboratorio di Irene Bozzoni, dove ha completato il dottorato di ricerca nel 2016. Ha poi vinto una EMBO fellowship e si è unito al laboratorio di Nikolaus Rajewsky presso il Max Delbrü Center – Berlin Institute for Medical Systems Biology, dove ha stabilito diverse linee di ricerca, ad esempio sviluppando FLAM-seq per lo studio della regolazione della coda di poli(A) e combinando optogenetica e trascrittomica spaziale per studiare la regolazione spaziale dell’espressione genica nel neurosviluppo usando organoidi. Si è trasferito a Milano e ha avviato il suo laboratorio ad HT a marzo 2023, lavorando nei campi della regolazione genica e del metabolismo dell’RNA, nonché sullo sviluppo di nuove tecnologie genomiche per la perturbazione e la profilazione dell’espressione genica.
E-mail: ivano.legnini [at] fht.org
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Pubblicazioni
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09/2023 - Nature Methods
Spatiotemporal, optogenetic control of gene expression in organoids
Organoids derived from stem cells have become an increasingly important tool for studying human development and modeling disease. However, methods are still needed to control and study spatiotemporal patterns of gene expression in organoids. Here we combined optogenetics and gene perturbation technologies to activate or knock-down RNA of target genes in programmable spatiotemporal patterns. To […]
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06/2023 - Nature Microbiology
Modelling viral encephalitis caused by herpes simplex virus 1 infection in cerebral organoids
Herpes simplex encephalitis is a life-threatening disease of the central nervous system caused by herpes simplex viruses (HSVs). Following standard of care with antiviral acyclovir treatment, most patients still experience various neurological sequelae. Here we characterize HSV-1 infection of human brain organoids by combining single-cell RNA sequencing, electrophysiology and immunostaining. We observed strong perturbations of […]
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05/2023 - Cell
Sites of transcription initiation drive mRNA isoform selection
The generation of distinct messenger RNA isoforms through alternative RNA processing modulates the expression and function of genes, often in a cell-type-specific manner. Here, we assess the regulatory relationships between transcription initiation, alternative splicing, and 3′ end site selection. Applying long-read sequencing to accurately represent even the longest transcripts from end to end, we quantify […]
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05/2023 - BioRxiv
High-resolution molecular atlas of a lung tumor in 3D
Cells live and interact in three-dimensional (3D) cellular neighborhoods. However, histology and spatial omics methods mostly focus on 2D tissue sections. Here we present a 3D spatial atlas of a routine clinical sample, an aggressive human lung carcinoma, by combining in situ quantification of 960 cancer-related genes across ∼340,000 cells with measurements of tissue-mechanical components. 3D cellular […]
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12/2022 - iScience
Rapid nuclear deadenylation of mammalian messenger RNA
Poly(A) tails protect RNAs from degradation and their deadenylation rates determine RNA stability. Although poly(A) tails are generated in the nucleus, deadenylation of tails has mostly been investigated within the cytoplasm. Here, we combined long-read sequencing with metabolic labeling, splicing inhibition and cell fractionation experiments to quantify, separately, the genesis and trimming of nuclear and […]
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