Ivano Legnini
- Research Group Leader, Legnini Group
Ivano Legnini è un biologo molecolare e dei sistemi interessato alla regolazione genica.
Si è laureato in Genetica e Biologia Molecolare all’Università La Sapienza di Roma nel 2012. Ha lavorato sull’RNA non codificante e la regolazione post-trascrizionale dell’espressione genica nel laboratorio di Irene Bozzoni, dove ha completato il dottorato di ricerca nel 2016. Ha poi vinto una EMBO fellowship e si è unito al laboratorio di Nikolaus Rajewsky presso il Max Delbrü Center – Berlin Institute for Medical Systems Biology, dove ha stabilito diverse linee di ricerca, ad esempio sviluppando FLAM-seq per lo studio della regolazione della coda di poli(A) e combinando optogenetica e trascrittomica spaziale per studiare la regolazione spaziale dell’espressione genica nel neurosviluppo usando organoidi. Si è trasferito a Milano e ha avviato il suo laboratorio ad HT a marzo 2023, lavorando nei campi della regolazione genica e del metabolismo dell’RNA, nonché sullo sviluppo di nuove tecnologie genomiche per la perturbazione e la profilazione dell’espressione genica.
E-mail: ivano.legnini [at] fht.org
Segui su Bluesky: @ilegnini.bsky.social
Pubblicazioni
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07/2026 - Nature Methods
SpatioTemporal Omics Consortium: a global effort for biological discovery across species, space and time
In this Comment we introduce the SpatioTemporal Omics Consortium (STOC), a global, open network collaborating to integrate spatial and temporal omics across species, development, disease and evolution. The pace of innovation in spatial technologies has been extraordinary, rapidly transforming our ability to study biological systems within their native tissue environments. Throughout the history of biology, […]
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07/2026 - BioRxiv
Insulin synthesis is sustained by Tent5 poly(A) polymerases
Insulin is an essential regulator of glucose homeostasis in vertebrates, and impairment of its synthesis or action leads to diabetes with severe health complications in humans. It is therefore essential to understand how beta cells control insulin synthesis and secretion, including the transcription, translation and decay of its messenger RNA. Using sequencing-based poly(A) tail length […]
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06/2026 - BioRxiv
Genomic codes governing enhancer RNA fate
Long-read sequencing has transformed transcriptome profiling, yet capturing full-length, non-polyadenylated transcripts like enhancer RNAs (eRNAs) remains challenging. Here, we introduce CFC-seq, combining cap-trapping and in vitro poly(A)-tailing to sequence poly(A) and non-poly(A) RNAs with precise transcription start site. Paired with our assembler, SALA, we identified 39,425 novel transcriptional units, including ∼24,000 eRNAs. Our data reveal […]
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04/2026 - BioRxiv
MicroRNAs are required within a critical time window to define neural patterning during early human brain development
MicroRNAs (miRNAs) are key post-transcriptional regulators of cell state transitions, yet their function in early human brain development is largely unknown. Here, we present a longitudinal analysis of miRNA function in developing human forebrain organoids. We show that mRNAs and miRNAs expression mirrors known developmental gene programs and that miRNA biogenesis peaks at neural commitment. […]
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01/2026 - BioRxiv
Genome Architecture Shapes Transcriptional Responses to DNA Supercoiling in a Multicellular Organism
DNA supercoiling is an intrinsic consequence of transcription that must be resolved to maintain proper gene expression. How DNA supercoiling shapes transcription dynamics in chromatinized animal genomes remains unclear. Here, we acutely depleted topoisomerases I and II in Caenorhabditis elegans and applied nascent transcription profiling, nuclear and total RNA-seq, histone modification mapping, and long-read sequencing […]
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