Minimal genome-wide human CRISPR-Cas9 library


  • Gonçalves E.,
  • Thomas M.,
  • Behan F. M.,
  • Picco G.,
  • Pacini C.,
  • Allen F.,
  • Vinceti A.,
  • Sharma M.,
  • Jackson D. A.,
  • Price S.,
  • Beaver C. M.,
  • Dovey O.,
  • Parry-Smith D.,
  • Iorio F.,
  • Parts L.,
  • Yusa K.,
  • Garnett M. J.


CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.

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