HT Course – BrainOmics


BrainOmics – computational approaches to single-cell multi-omics in neuroscience
Location: Human Technopole, Milan, Italy
Date: Nov 28 – Dec 2, 2022
Target audience: 20 participants selected through application
Deadline for registrations: 30 September 2022
Registration fee: 650 €
Course Overview
Single-cell omics technologies are breaking new ground in neurobiology by substantially increasing the precision and resolution with which the complex cell populations of the nervous system can be characterised. Approaches that profile several layers of information (genome, epigenome, transcriptome, proteome) allow to generate data of unprecedented depth on the molecular state of the diversity of cells composing the nervous system.
This increase in data volume and complexity generates as many opportunities as new analytical challenges.
This compact course aims at empowering participants in mastering key computational tools for the analysis of single cell multi-omics datasets, starting from individual molecular layers to then tackle their integration, alongside providing a theoretical overview of the impact of these technologies at the leading edge of neurobiology.
Target Audience
This course is aimed at bioinformaticians and computational biologists with (at least) a basic knowledge of omics techniques.
The core of the course will be centred around hands-on data analysis sessions. A basic understanding of Unix command line, python and/or R is required. Previous experience with single-cell analytical workflow will be considered as an added value to harness the most from the course structure.
Main Topics
Lectures: impact of multimodal approaches on neurobiology research; innovative computational approaches for multi-omics data analysis and integration.
Computational hand-on sessions: key steps and troubleshooting in the analysis of single-cell transcriptomics data; multiplexing approaches and demultiplexing algorithms; analytical workflow for single cell ATACSeq; multi-omics data integration.
Wet Lab demo session: technologies for multimodal single-cell interrogation in neural tissues.
Roundtable with core facilities: structuring integrated multi-omic/functional workflows for neural tissue.
Scientific Organisers
-
Giuseppe Testa
Head of Neurogenomics Research Centre -
Jose Davila-Velderrain
Research Group Leader -
Elena Taverna
Research Group Leader
External Speakers and Instructors
- J. Gray Camp – Group Leader Roche Institute for Translational Bioengineering; Assistant Professor, University of Basel, Switzerland
- Denis Jabaudon – Professor, University of Geneva, Switzerland
- Peter Kharchenko – Principal Investigator, Atlos Labs, US
- Gioele La Manno – Head of the Neurodevelopmental Systems Biology lab, École Polytechnique Fédérale de Lausanne, Switzerland
- Shahin Mohammadi – Sr. Data Scientist, Insitro, US
- Genevieve Stein-O’Brien – Assistant Professor, Department of Neuroscience, Johns Hopkins University, School of Medicine, Baltimore, US
- Bosiljka Tasic – Director, Molecular Genetics, Allen Institute for Brain Science, Seattle, US
- Joshua Welch – Assistant Professor, Department of Computational Medicine and Bioinformatics University of Michigan, US
HT Speakers and Instructors – Neurogenomics Research Centre
- Daniele Capocefalo – Postdoc
- Nicolò Caporale – Postdoc
- Davide Castaldi – PhD Student
- Cristina Cheroni – Postdoc
- Jose Davila-Velderrain – Research Group Leader
- Elena Taverna – Research Group Leader
- Giuseppe Testa – Head of the Neurogenomics Research Centre; Professor of Molecular Biology at the University of Milan and Group Leader at IEO, Milan, Italy
- Emanuele Villa – Staff Scientist
- Alessandro Vitriolo – Postdoc
- Asia Zonca – PhD student
HT Facilities and Support Units
- Genomics Technologies Development Unit, Genomics Research Centre (Nicola Crosetto, Senior Manager)
- Automated Stem Cell and Organoid Facility (Giovanni Faga, Head)
- Image Analysis Facility (Florian Jug, Head)
- Light Imaging Facility (Nicola Maghelli, Senior Manager)
- Genomics Facility (Clelia Peano, Senior Manager)
- Electrophysiology Scientific Service, Neurogenomics Research Centre (Diletta Pozzi, Manager)
Contact: training@fht.org